addgene cat 110164 software (Addgene inc)
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addgene cat 110164 software
Addgene Cat 110164 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px458+ruby/pX458_Ruby+(Plasmid+%23110164)/pm41734765-267-241-241
Average 93 stars, based on 15 article reviews
Addgene Cat 110164 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px458+ruby/pX458_Ruby+(Plasmid+%23110164)/pm41734765-267-241-241
Average 93 stars, based on 15 article reviews
addgene cat 110164 software - by Bioz Stars,
2026-09
93/100 stars
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Clone Assay:Article Title: GTP Cyclohydrolase Drives Breast Cancer Development and Promotes EMT in an Enzyme-Independent Manner Article Snippet: GTP cyclohydrolase (GCH1) is the rate-limiting enzyme for tetrahydrobiopterin (BH4) biosynthesis.. The catalysis of BH4 biosynthesis is tightly regulated for physiological neurotransmission, inflammation, and vascular tone.. Paradoxically, BH4 has emerged as an oncometabolite regulating tumor growth, but the effects on tumor development remain controversial. Article Title: Annexin A2 mediates RIG-I-like receptor responses to viral infection Article Snippet: THP1 cells were differentiated into macrophage-like cells prior to experiments by 24 h treatment with 10 ng/ml phorbol myristate acetate (PMA; Invivogen). .. For generation of ANXA2 knockout cells using CRISPR/Cas9 technology, two sgRNAs targeting upstream and downstream of exon 2 of the human ANXA2 gene were cloned into either pX458-eGFP or Article Title: Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Article Snippet: .. In particular, the sgRNA pairs targeted defined critical exons of PEX5 (exon 2) and PEX14 (exon 3), resulting in nonsense-mediated mRNA decay (NMD), the likely event after homozygous deletion in both cases. sgRNAs were selected using the CRISPOR algorithm ( ). sgRNAs targeting the intron upstream of the respective splice branch point were cloned into pX458 (Addgene 48138, Dr. Feng Zhang), and sgRNAs targeting the intron downstream of the target exon were cloned into Article Title: Super-enhancers require a combination of classical enhancers and novel facilitator elements to drive high levels of gene expression Article Snippet: Candidates with the fewest predicted off-targets were selected and further screened for their effectiveness, using an in vitro surveyor assay (according to the manufacturer, IDT). .. For enhancer deletion, gRNAs were designed flanking the targeted enhancer, and cloned into pSpCas9 (BB)-2A-GFP (pX458) vector, a gift from Feng Zhang (Addgene plasmid: #48138), or Article Title: Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Article Snippet: .. In particular, the sgRNA pairs targeted defined critical exons of PEX5 (exon 2) and PEX14 (exon 3), resulting in nonsense-mediated mRNA decay, the likely event after homozygous deletion in both cases. sgRNAs were selected using the CRISPOR algorithm ( ). sgRNAs targeting the intron upstream of the respective splice branch point were cloned into pX458 (Addgene 48138, Dr. Feng Zhang), and sgRNAs targeting the intron downstream of the target exon were cloned into Article Title: ATRX loss couples genome instability at a G-rich repeat to dysregulation of human alpha-globin expression. Article Snippet: .. The dual gRNAs were cloned into the pX458-eGFP (Addgene 48138) and Article Title: ATRX loss couples genome instability at a G-rich repeat to dysregulation of human alpha-globin expression Article Snippet: .. The dual gRNAs were cloned into the pX458-eGFP (Addgene 48138) and Activity Assay:Article Title: Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions Article Snippet: .. Individual ssODNs for sgRNA pairs were subcloned into pX458-eGFP (Addgene 48138) and Knock-Out:Article Title: Annexin A2 mediates RIG-I-like receptor responses to viral infection Article Snippet: THP1 cells were differentiated into macrophage-like cells prior to experiments by 24 h treatment with 10 ng/ml phorbol myristate acetate (PMA; Invivogen). .. For generation of ANXA2 knockout cells using CRISPR/Cas9 technology, two sgRNAs targeting upstream and downstream of exon 2 of the human ANXA2 gene were cloned into either pX458-eGFP or CRISPR:Article Title: Annexin A2 mediates RIG-I-like receptor responses to viral infection Article Snippet: THP1 cells were differentiated into macrophage-like cells prior to experiments by 24 h treatment with 10 ng/ml phorbol myristate acetate (PMA; Invivogen). .. For generation of ANXA2 knockout cells using CRISPR/Cas9 technology, two sgRNAs targeting upstream and downstream of exon 2 of the human ANXA2 gene were cloned into either pX458-eGFP or Mutagenesis:Article Title: Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Article Snippet: .. In particular, the sgRNA pairs targeted defined critical exons of PEX5 (exon 2) and PEX14 (exon 3), resulting in nonsense-mediated mRNA decay (NMD), the likely event after homozygous deletion in both cases. sgRNAs were selected using the CRISPOR algorithm ( ). sgRNAs targeting the intron upstream of the respective splice branch point were cloned into pX458 (Addgene 48138, Dr. Feng Zhang), and sgRNAs targeting the intron downstream of the target exon were cloned into Article Title: Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Article Snippet: .. In particular, the sgRNA pairs targeted defined critical exons of PEX5 (exon 2) and PEX14 (exon 3), resulting in nonsense-mediated mRNA decay, the likely event after homozygous deletion in both cases. sgRNAs were selected using the CRISPOR algorithm ( ). sgRNAs targeting the intron upstream of the respective splice branch point were cloned into pX458 (Addgene 48138, Dr. Feng Zhang), and sgRNAs targeting the intron downstream of the target exon were cloned into Plasmid Preparation:Article Title: Super-enhancers require a combination of classical enhancers and novel facilitator elements to drive high levels of gene expression Article Snippet: Candidates with the fewest predicted off-targets were selected and further screened for their effectiveness, using an in vitro surveyor assay (according to the manufacturer, IDT). .. For enhancer deletion, gRNAs were designed flanking the targeted enhancer, and cloned into pSpCas9 (BB)-2A-GFP (pX458) vector, a gift from Feng Zhang (Addgene plasmid: #48138), or |